Journal: Journal of Cardiovascular Magnetic Resonance

Article Title: A prospective evaluation of cardiovascular magnetic resonance measures of dyssynchrony in the prediction of response to cardiac resynchronization therapy

doi: 10.1186/s12968-014-0058-0

Figure Lengend Snippet: Representative figure from the TomTec platform used to determine volume-change SDI. (A and B) The software requires endocardial contours to be outlined at end-diastole and end-systole in the 4-chamber, 3-chamber and 2-chamber (not shown). The software then produces a 3-dimensional shell of the endocardial cavity that tracks the endocardial contours throughout the cardiac cycle (C) . Time-volume curves are then generated that represent the time to reach minimum volume for each of the 16 segments of the LV (D) .

Article Snippet: Volume SDI (derived from TomTec 4D LV-Analysis software) intra- and inter-observer agreements were assessed according to the statistical methods proposed by Bland and Altman [ ].

Techniques: Software, Generated

Journal: Journal of Translational Medicine

Article Title: Silencing PinX1 enhances radiosensitivity and antitumor-immunity of radiotherapy in non-small cell lung cancer

doi: 10.1186/s12967-024-05023-y

Figure Lengend Snippet: Silencing PinX1 combined with IR increased CD8 + T cell recruitment. A Lewis cells were transfected with a control or shPinX1 lentivirus and analyzed by western blotting. B. Experimental schedule of varied treatment regimens for LLC tumor-bearing mice. C Representative photos of tumor xenografts 14 days after the initiation of IR. D Tumor volume of xenografts was shown in the graphs. Two-way ANOVA with Tukey’s multiple comparison test was conducted for tumor volume analysis with GraphPad Prism 8.0.2. n = 5 mice/group. E Survival data were compared using log-rank Mantel-Cox test. n = 8 mice/group. F – K Representative flow cytometry staining and frequencies of total CD8 + /CD3 + cells, PD-1 + Tim-3 + /CD8 + T cells, and Gzmb + /CD8 + T cells in tumors. n = 5 mice/group. Data are presented as the mean ± standard deviation, *: p < 0.05, **: p < 0.01, *** p : < 0.001, ****: p < 0.0001

Article Snippet: Two-way ANOVA with Tukey’s multiple comparison was conducted for tumor volume analysis with GraphPad Prism 8.0.2. n = 5 mice/group.

Techniques: Transfection, Western Blot, Comparison, Flow Cytometry, Staining, Standard Deviation

Journal: Journal of Translational Medicine

Article Title: Silencing PinX1 enhances radiosensitivity and antitumor-immunity of radiotherapy in non-small cell lung cancer

doi: 10.1186/s12967-024-05023-y

Figure Lengend Snippet: Silencing PinX1 potentiates the antitumor efficacy of radioimmunotherapy. A Experimental schedule of varied treatment regimens for LLC tumor-bearing mice. B Representative photos of tumor xenografts 14 days after the initiation of IR. C Tumor volume of xenografts was shown in the graphs. Two-way ANOVA with Tukey’s multiple comparison was conducted for tumor volume analysis with GraphPad Prism 8.0.2. n = 5 mice/group. D , E Representative flow cytometry staining and frequencies of total CD8 + /CD3 + cells in tumors. n = 5 mice/group. F , G Representative flow cytometry staining and frequencies of PD-1 + Tim-3 + /CD8 + T cells in tumors. n = 5 mice/group. H – J . Representative flow cytometry staining and frequencies of CD86 + CD206 − /CD45 + F4/80 + CD11b + cells and CD86 − CD206 + /CD45 + F4/80 + CD11b + cells cells in tumors. n = 5 mice/group. Data are presented as the mean ± standard deviation. *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001

Article Snippet: Two-way ANOVA with Tukey’s multiple comparison was conducted for tumor volume analysis with GraphPad Prism 8.0.2. n = 5 mice/group.

Techniques: Comparison, Flow Cytometry, Staining, Standard Deviation